FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309
Sequences flagged as poor quality0
Sequence length249-251
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT216.796116504854369No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT154.854368932038835No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC123.8834951456310676No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC113.559870550161812No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT82.5889967637540456No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT82.5889967637540456No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC82.5889967637540456No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT82.5889967637540456No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT72.26537216828479No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT72.26537216828479No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT61.9417475728155338No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT61.9417475728155338No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC51.6181229773462782No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC51.6181229773462782No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT51.6181229773462782No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT51.6181229773462782No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC51.6181229773462782No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT51.6181229773462782No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT41.2944983818770228No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT41.2944983818770228No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT41.2944983818770228No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT41.2944983818770228No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT41.2944983818770228No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT41.2944983818770228No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGGAACCCTGAT30.9708737864077669No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC30.9708737864077669No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT30.9708737864077669No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT30.9708737864077669No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT30.9708737864077669No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC30.9708737864077669No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT30.9708737864077669No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT30.9708737864077669No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT30.9708737864077669No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT30.9708737864077669No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT30.9708737864077669No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT30.9708737864077669No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA20.6472491909385114No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT20.6472491909385114No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT20.6472491909385114No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA20.6472491909385114No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT20.6472491909385114No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT20.6472491909385114No Hit
CTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGATC20.6472491909385114No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT20.6472491909385114No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT20.6472491909385114No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT20.6472491909385114No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT20.6472491909385114No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT20.6472491909385114No Hit
CCTACGGGAGGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGA20.6472491909385114No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT20.6472491909385114No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT20.6472491909385114No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGACGAGAGTCTGAA20.6472491909385114No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGCCATTGGGGGCAACCCTGAT10.3236245954692557No Hit
CCTACGGGCGGCGGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA10.3236245954692557No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA10.3236245954692557No Hit
CCTCCGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA10.3236245954692557No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACCATGGGGGCAACCCTGCT10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGCGCAAGCCTGAA10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGAGGGAACCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTCGTCAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGGGGCAACCCTGAC10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTAGGGAATCATCCGCAATGGACGAAAGTCTGAC10.3236245954692557No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10.3236245954692557No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10.3236245954692557No Hit
ACTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA10.3236245954692557No Hit
CCTACGGGTGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA10.3236245954692557No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACATTGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTCCGCAATGGACGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT10.3236245954692557No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT10.3236245954692557No Hit
CCTACGGGAGGCATCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCTCAATGGGCGAAAGCCTGAA10.3236245954692557No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACATTGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGTCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA10.3236245954692557No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGTCAATGGGCGAGAGCCTGAA10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA10.3236245954692557No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGCCTGAA10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGCCTGAA10.3236245954692557No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGCACAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGCTTAAC10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACCATGGGGGCAACCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGACGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10.3236245954692557No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTGCACAATGGACGAAAGTCTGAT10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTAGGGAATCATCCGCAATGGACGAAAGCCTGAC10.3236245954692557No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAAGCCTGAA10.3236245954692557No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAAAGCCTGAA10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAC10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGCACAATGGGCGAAAGCCTGAA10.3236245954692557No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT10.3236245954692557No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT10.3236245954692557No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAGGTCTGAC10.3236245954692557No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGCCTGAA10.3236245954692557No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA10.3236245954692557No Hit
CCTACGGGGGGCTGCAGTAGGGGATCTTCCACAATGGACGAAAGTCTGAT10.3236245954692557No Hit
CCTACGGGTGGCGGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10.3236245954692557No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGCAACCCTGAT10.3236245954692557No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAACCCTGAT10.3236245954692557No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGACAATGGGGGCAACCCTGAT10.3236245954692557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)