FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309
Sequences flagged as poor quality0
Sequence length248-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG227.119741100323624No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG216.796116504854369No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG134.207119741100324No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG123.8834951456310676No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG113.559870550161812No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG82.5889967637540456No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG72.26537216828479No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA72.26537216828479No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG72.26537216828479No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG61.9417475728155338No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG61.9417475728155338No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG61.9417475728155338No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG51.6181229773462782No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA51.6181229773462782No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG51.6181229773462782No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG51.6181229773462782No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG51.6181229773462782No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA51.6181229773462782No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG51.6181229773462782No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG41.2944983818770228No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA41.2944983818770228No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG41.2944983818770228No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG41.2944983818770228No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG41.2944983818770228No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG30.9708737864077669No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30.9708737864077669No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG30.9708737864077669No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG30.9708737864077669No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30.9708737864077669No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG30.9708737864077669No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA30.9708737864077669No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG20.6472491909385114No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20.6472491909385114No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20.6472491909385114No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG20.6472491909385114No Hit
AACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20.6472491909385114No Hit
GACACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGC20.6472491909385114No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20.6472491909385114No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG20.6472491909385114No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA20.6472491909385114No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA20.6472491909385114No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20.6472491909385114No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20.6472491909385114No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20.6472491909385114No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG20.6472491909385114No Hit
GACTACCCGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG20.6472491909385114No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG20.6472491909385114No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG20.6472491909385114No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20.6472491909385114No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG20.6472491909385114No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA10.3236245954692557No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.3236245954692557No Hit
GACTACTCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.3236245954692557No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.3236245954692557No Hit
GACTACCAGGGTATCTAATCCCGTTTGATACCCTGGCTTTCGAGCCTCAA10.3236245954692557No Hit
GCCTACACGGGTATTTATTCCTGTTTGATTCCTACGTTTTCGGTCATCAG10.3236245954692557No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCTCATGCTTTCGCTCCTCAG10.3236245954692557No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGAATGAG10.3236245954692557No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10.3236245954692557No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACACCTTCGAGCCTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGATCATCAG10.3236245954692557No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG10.3236245954692557No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTTCATCAG10.3236245954692557No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGAGCATCAG10.3236245954692557No Hit
GACTACCAGGGTATCTAATCCTGTTTGCCCCCCACGCTTTCGTGCATGAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG10.3236245954692557No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10.3236245954692557No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCTTCAG10.3236245954692557No Hit
GACTACAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGC10.3236245954692557No Hit
GACTACTAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCA10.3236245954692557No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG10.3236245954692557No Hit
ACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAGC10.3236245954692557No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAA10.3236245954692557No Hit
GACTACTAGGGTATCTAATTCTGTTTGCTCCCCACACTTTAGCACCTGAG10.3236245954692557No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG10.3236245954692557No Hit
GACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10.3236245954692557No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10.3236245954692557No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCATCAG10.3236245954692557No Hit
GACTACAAGGTTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10.3236245954692557No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCATCAG10.3236245954692557No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTGGCGTGCCTCAG10.3236245954692557No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTCAG10.3236245954692557No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10.3236245954692557No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.3236245954692557No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACACTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCATCAA10.3236245954692557No Hit
GACGACACGGGGATCTAATCAAGTCCAGTATCGAGCTTATAGAGAGACTG10.3236245954692557No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCGCCCCAG10.3236245954692557No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10.3236245954692557No Hit
GACTACTAGGGTTCTTATCCTGGTTGGCTCCCCCCGCTTTCGGGCCCAGG10.3236245954692557No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10.3236245954692557No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCAAGCTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTCGCATTCGCGCTTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG10.3236245954692557No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG10.3236245954692557No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCCGTTTGCTCCCCAGGCCTTCGAGCCTCAG10.3236245954692557No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACACTTTCGAGCATCAG10.3236245954692557No Hit
TACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA10.3236245954692557No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACAAGGGTATCTAATCCTAGTTTGAACCTTCACTATCGAGTATTCA10.3236245954692557No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTGCGTTACCCGCACCTTCGCGCATAAG10.3236245954692557No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGAGCCTCAG10.3236245954692557No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG10.3236245954692557No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAA10.3236245954692557No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAA10.3236245954692557No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10.3236245954692557No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCATCAG10.3236245954692557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)