FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133
Sequences flagged as poor quality0
Sequence length250-251
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG75.263157894736842No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG64.511278195488721No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG64.511278195488721No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG43.007518796992481No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA32.2556390977443606No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG32.2556390977443606No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG21.5037593984962405No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21.5037593984962405No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21.5037593984962405No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21.5037593984962405No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21.5037593984962405No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21.5037593984962405No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21.5037593984962405No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21.5037593984962405No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA21.5037593984962405No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21.5037593984962405No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG21.5037593984962405No Hit
GACTACAGGGGTATCTAATCCTGTTCGATCCCCATACTTTCGCTCCTCAG10.7518796992481203No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10.7518796992481203No Hit
GACTACTAGGGTATCTAATCCTGTCCAGTATCCGGCTTTTCGAGCGACTG10.7518796992481203No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10.7518796992481203No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10.7518796992481203No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10.7518796992481203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCATGAG10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCGCTTTCGAGCATGAG10.7518796992481203No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA10.7518796992481203No Hit
GACTACCCGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGC10.7518796992481203No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.7518796992481203No Hit
GACTACCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGC10.7518796992481203No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG10.7518796992481203No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACCCTTTCGCTCCTCAG10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTGCCTCAG10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10.7518796992481203No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTGAC10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCATGAG10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10.7518796992481203No Hit
GATTCATCGGGTTCTTAATCCTGTTCGTCCCCAACGTTTCCCGCCTCAGC10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCGCTTTCGTGCATGAG10.7518796992481203No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10.7518796992481203No Hit
GACTACTAGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAGC10.7518796992481203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10.7518796992481203No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCATCAG10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG10.7518796992481203No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG10.7518796992481203No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG10.7518796992481203No Hit
GACTACCGGGGAATCTAATCCTGTCGACTCCCGCCGCTTTGGTCCACAGG10.7518796992481203No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGATCCTCAG10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAG10.7518796992481203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.7518796992481203No Hit
GACTACCGGGTATCTTATCCTGTTTGCATCCCACGGTTTCGCGCCTCAGG10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10.7518796992481203No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCAAGCTTTCGCTCCTTACC10.7518796992481203No Hit
GACTACTCGGGTATCTAAGCCTGTTTGATCCCCACCCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCAG10.7518796992481203No Hit
GACTACCCGGTTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG10.7518796992481203No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.7518796992481203No Hit
ACTACCAGGGTATCTAATCCTGTTCGATACCCGAACTTTCGAGCTTCAGC10.7518796992481203No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG10.7518796992481203No Hit
GACTACCCGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG10.7518796992481203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTCGCTTTTGAGCCTCCA10.7518796992481203No Hit
GACTACCAGGGTACCTGATCCTGTTTGCTCCCCACGCTATCGAGCCTCAA10.7518796992481203No Hit
GACTACCCGGGTATTTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG10.7518796992481203No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGAGCATCAG10.7518796992481203No Hit
GACTACCGGGGTATCTAATCCTGTTTGATAGCCACTCTTTCAAGGATCGG10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.7518796992481203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAC10.7518796992481203No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10.7518796992481203No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC10.7518796992481203No Hit
GCCTACTAGGGTACCTAATCCTGTTCGATACCAGCACTTTCGAGCTTCAG10.7518796992481203No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10.7518796992481203No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG10.7518796992481203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG10.7518796992481203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGATTTCGCGCCTCAG10.7518796992481203No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC10.7518796992481203No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGTGCCTCAG10.7518796992481203No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTAAG10.7518796992481203No Hit
GACTACCCGGGTATCTAAGCCTGTTTGCTCCCCACGCTTACGCCCCCGAG10.7518796992481203No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG10.7518796992481203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)