FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168
Sequences flagged as poor quality0
Sequence length35-251
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG116.547619047619048No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG84.761904761904762No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG63.571428571428571No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG63.571428571428571No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG63.571428571428571No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG42.380952380952381No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG42.380952380952381No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG42.380952380952381No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31.7857142857142856No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG31.7857142857142856No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31.7857142857142856No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31.7857142857142856No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31.7857142857142856No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG31.7857142857142856No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG31.7857142857142856No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG21.1904761904761905No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21.1904761904761905No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21.1904761904761905No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG21.1904761904761905No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG21.1904761904761905No Hit
GACTACCAGGGTATCAAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21.1904761904761905No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21.1904761904761905No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21.1904761904761905No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21.1904761904761905No Hit
GACTACACGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG21.1904761904761905No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA21.1904761904761905No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21.1904761904761905No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAA21.1904761904761905No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG21.1904761904761905No Hit
GACTACTGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21.1904761904761905No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG10.5952380952380952No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCGCCTCAA10.5952380952380952No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG10.5952380952380952No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.5952380952380952No Hit
GACTACTCGGGTTTTCTAACCTGTTCGATACCCCCGCTTTCGGGCCCCAG10.5952380952380952No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG10.5952380952380952No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10.5952380952380952No Hit
GACTACTAGGCTTCCGGGTCCCTTTAGCTCACCAACCTGACGATCATCAT10.5952380952380952No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10.5952380952380952No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTGCCTCAG10.5952380952380952No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAACCTCAG10.5952380952380952No Hit
GACTACACGGGTATCTAATCCTGTATGCTCCTCACGCATTCGAGCCTCAG10.5952380952380952No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG10.5952380952380952No Hit
GACTACAGGGGTATCTAATCCTGGTTGCTCCCCACGCCTTCGCGCCTCAA10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCTTCAG10.5952380952380952No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG10.5952380952380952No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG10.5952380952380952No Hit
NCTACAGGGGTATTTTATCCCGTTTGCTCCCCAACACTTTGCACCTAAGC10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG10.5952380952380952No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC10.5952380952380952No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCATGAG10.5952380952380952No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTGAG10.5952380952380952No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG10.5952380952380952No Hit
GACTACCAGGGTGTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10.5952380952380952No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG10.5952380952380952No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10.5952380952380952No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAA10.5952380952380952No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10.5952380952380952No Hit
GACTACTCGGGTATCTAATCCTGTTTGCCCCCCACGCTCTCGCACCTGAG10.5952380952380952No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCATGAA10.5952380952380952No Hit
GACTACCGGGGTACTCAACCCTTTTCATTCCCACAGCTTCCGGCCATCAT10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10.5952380952380952No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10.5952380952380952No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCGTTCGTGCATGAG10.5952380952380952No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10.5952380952380952No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.5952380952380952No Hit
GACTACCCGGGTATCTTCGGGAGGGCAAGAAGCAGGTCACGGTGGCCGTT10.5952380952380952No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10.5952380952380952No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG10.5952380952380952No Hit
GACTACCAGGGTATCTAAGCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10.5952380952380952No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.5952380952380952No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.5952380952380952No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.5952380952380952No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCAGGCTTTCGCGCCTCAC10.5952380952380952No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAA10.5952380952380952No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGAGCCTCAG10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG10.5952380952380952No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCAAACTTTCGAGCCTCAA10.5952380952380952No Hit
GACTCCTTGGGTATCTTATCCTGGTTGCCACTTCTGCTTCCCCGCCTCAG10.5952380952380952No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCATCAG10.5952380952380952No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCATCAG10.5952380952380952No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.5952380952380952No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCCCGCTTTCGCGCCTCAG10.5952380952380952No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCATGAG10.5952380952380952No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC10.5952380952380952No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10.5952380952380952No Hit
GACTACAAGGGTATCTAATTCTGTTTGCTCCCCACGCTTTCGTGCCTGAG10.5952380952380952No Hit
GACTACCCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.5952380952380952No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG10.5952380952380952No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10.5952380952380952No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10.5952380952380952No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGTCCCTCAG10.5952380952380952No Hit
GCTACCTAGGGTACTCAATCCTGTTTGTACCCCAAGCTTTCGTCCCCCAG10.5952380952380952No Hit
GAAGCTCCGGTTCTCTGCTTCTATTTGCCCGGGCGACCGTGTGAGATTTC10.5952380952380952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)