FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114
Sequences flagged as poor quality0
Sequence length249-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG54.385964912280701No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG54.385964912280701No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG43.508771929824561No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG43.508771929824561No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG43.508771929824561No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG32.631578947368421No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG32.631578947368421No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG32.631578947368421No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG32.631578947368421No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG32.631578947368421No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21.7543859649122806No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21.7543859649122806No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21.7543859649122806No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA21.7543859649122806No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21.7543859649122806No Hit
NACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21.7543859649122806No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTGCCCACGCTTTCGTGCATGAG21.7543859649122806No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21.7543859649122806No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG21.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG21.7543859649122806No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAA21.7543859649122806No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG10.8771929824561403No Hit
GACTACACGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGAGCTTCAG10.8771929824561403No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.8771929824561403No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10.8771929824561403No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGATCATCAG10.8771929824561403No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10.8771929824561403No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCATCAG10.8771929824561403No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGATCCTCAG10.8771929824561403No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTGAG10.8771929824561403No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10.8771929824561403No Hit
GACTACCCGGGTATCTAATCCTGGATGGCTCCCTGACATCAGTCCCTTTG10.8771929824561403No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGAGCATCAG10.8771929824561403No Hit
GACTACCCGGGTATCTAATCCTGGAAGGTTGTCTTACATTCCCCCGTCTG10.8771929824561403No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG10.8771929824561403No Hit
GACTACTGGGGTACTTATCCCTGGTTCCTCCCCACCTTTCCGTCCCTGGG10.8771929824561403No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTTAG10.8771929824561403No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10.8771929824561403No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGCGCCTCAG10.8771929824561403No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCAGGCTTTCGCGCCTCAG10.8771929824561403No Hit
ACCACCCGGGTATCCAATCCTGTTTGATACCCACACTTTCGAGCATCAGC10.8771929824561403No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGTGCCTCAG10.8771929824561403No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGACCCTCAG10.8771929824561403No Hit
GACTACCAGGGTAGCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC10.8771929824561403No Hit
GACTACAAGGGTAGCTAATCCTGTTCGATACCCACGCTTTCATGCCTCAG10.8771929824561403No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGAGCATCAG10.8771929824561403No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10.8771929824561403No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG10.8771929824561403No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCACCTGAG10.8771929824561403No Hit
GCCTACCAGGGTATCTAAGCTGTGTGGTACCCACTCCTTCGGCCATTAGC10.8771929824561403No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.8771929824561403No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG10.8771929824561403No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG10.8771929824561403No Hit
GACTACTCGGGTATCTAATCCTGTTTGCCCCCCCCCCTTTCGTTCATGAG10.8771929824561403No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG10.8771929824561403No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGCGCCTCAG10.8771929824561403No Hit
GACACCAGGGTTCCCCGTGGCGATCTCTCTCCTAGACTACACTCCTCCGC10.8771929824561403No Hit
GACTACCCGGGTATCTAATCCTGGTTGCTCCCCACGCTTTCGAGCCTCAG10.8771929824561403No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10.8771929824561403No Hit
GACTACACGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG10.8771929824561403No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCAG10.8771929824561403No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG10.8771929824561403No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.8771929824561403No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10.8771929824561403No Hit
GAATCGTAGGGTCTCTGCCCGTAGATTTGTATCAGAAATACGACAAATAG10.8771929824561403No Hit
GACTACTGGGGTCTCTCTTCCTGTTTGTTCCCCACATTTTTGCACCTGCG10.8771929824561403No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCATCAG10.8771929824561403No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG10.8771929824561403No Hit
GCATCCAGGGGTATCTGACCCTGACAGGCAGTCTAACATCACTCCGATTG10.8771929824561403No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10.8771929824561403No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10.8771929824561403No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCGCCTCAG10.8771929824561403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)