FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length250-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG67.0588235294117645No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG55.88235294117647No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG44.705882352941177No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG33.5294117647058822No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG33.5294117647058822No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG33.5294117647058822No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG33.5294117647058822No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG33.5294117647058822No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG33.5294117647058822No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTTAG22.3529411764705883No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAT22.3529411764705883No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG22.3529411764705883No Hit
GACACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGC22.3529411764705883No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG22.3529411764705883No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG22.3529411764705883No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG22.3529411764705883No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG22.3529411764705883No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11.1764705882352942No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11.1764705882352942No Hit
GACTACACGGGTATCTAATCCCGGTTGGTCTCTTCGCTTTTGTGTATCAA11.1764705882352942No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTGAG11.1764705882352942No Hit
GACTACTAGGGTATCTGAGCCGGTTTGTTATCCACGTAATCGTACACGCG11.1764705882352942No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTAGCTCCTCAG11.1764705882352942No Hit
GACTACTAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11.1764705882352942No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGGGTCTCAG11.1764705882352942No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGTTTTCGAGCCTCAG11.1764705882352942No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAG11.1764705882352942No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG11.1764705882352942No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11.1764705882352942No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11.1764705882352942No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11.1764705882352942No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11.1764705882352942No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA11.1764705882352942No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG11.1764705882352942No Hit
GACTACCAGGGTATCTACGCCGGGTTTCTCACCACGATTTCGCACATGAG11.1764705882352942No Hit
GACTACCAGGGTATCTAATCCTTTTTCTACCCACCCCTTTATGCCTCCGC11.1764705882352942No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTCAG11.1764705882352942No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA11.1764705882352942No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG11.1764705882352942No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG11.1764705882352942No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA11.1764705882352942No Hit
GACTACAAGGGTATCTAATCCTTTTGGTTCCCACCACTTCATGCACCCAC11.1764705882352942No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA11.1764705882352942No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG11.1764705882352942No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11.1764705882352942No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTCGCTTTCGCGCCTCAG11.1764705882352942No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11.1764705882352942No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG11.1764705882352942No Hit
GACTACTAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG11.1764705882352942No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC11.1764705882352942No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG11.1764705882352942No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11.1764705882352942No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11.1764705882352942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)