FastQCFastQC Report
Fri 27 May 2022
EGAF00004836963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004836963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360863510
Sequences flagged as poor quality0
Sequence length74
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG11180030.30981325875813825Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA6846730.18973184625954562Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAAC4076120.11295461821562397No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC3962310.10980079421163975Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGA3881220.10755368421706034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)