FastQCFastQC Report
Fri 27 May 2022
EGAF00004836965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004836965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366961084
Sequences flagged as poor quality0
Sequence length74
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG10366800.2825040706496278Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA6336130.17266490307184726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAAC4584840.12494076892360607No Hit
GTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGA4297270.11710424313004263No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATTAAA4109810.11199579953279189No Hit
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGA4081840.11123359336926308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)