FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004838129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548299
Sequences flagged as poor quality0
Sequence length300
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAG25912147.25906850094565No Hit
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGA25580646.65447137419547No Hit
TTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGA25660.4679928287303095No Hit
GAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGAT16820.3067669282635934No Hit
TCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAA6770.12347277671489461No Hit
CAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAAA6750.12310801223420068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)