FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004838130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548299
Sequences flagged as poor quality0
Sequence length300
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGA24777945.19048913093039No Hit
TTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAG24331344.375970045540846No Hit
TTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGA31950.5827112579085499No Hit
GAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGAT21590.39376325690909525No Hit
TGAGACCATGGACTGACGACATGTGTACTCACGCCTACAGCTCCAGCTGA18720.34141955392951656No Hit
TTTCAGGTAGTTCATAATGCAATCATAATAGAGACGACTAATTTAGGAAG17900.32646421022106553No Hit
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGC9960.18165271138557612No Hit
TGAGACCATGGACTGACGACATGTGTACTCACGCGTACAGCTCCAGCTGA8920.16268495838949187No Hit
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCCCCAGCTGA7910.1442643521144485No Hit
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCGCCAGCTGA7880.14371720539340763No Hit
TTTCAGGTAGTTCATAATGCAATCATAATAGAGAGGACTAATTTAGGAAG7530.13733382698126387No Hit
TTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAAGTTAGGAAG7390.13478047561640638No Hit
CAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAAA7040.12839709720426265No Hit
TCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAA6670.12164895431142496No Hit
TGCGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGA5510.10049261443117714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)