FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004838147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329138
Sequences flagged as poor quality0
Sequence length300
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGA15385146.74361514015398No Hit
TTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAG15242646.310666042814866No Hit
TTGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTG12880.391325219208964No Hit
TTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGA8720.26493446517873964No Hit
GAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGAT8160.2479203252131325No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATCGGATCTCGTA6700.20356203173137102TruSeq Adapter, Index 7 (97% over 39bp)
TTTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAA5030.15282343576250693No Hit
TCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAA4570.1388475350764725No Hit
CAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAAA4510.13702459150872887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)