FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004838148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329138
Sequences flagged as poor quality0
Sequence length300
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAG15353946.648822074631305No Hit
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGA15227046.26326951005353No Hit
TTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGA18320.5566054360177191No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC9600.29167097083897936Illumina Single End PCR Primer 1 (100% over 50bp)
GAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGAT8590.26098475411529515No Hit
TTGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTG5940.1804714132066185No Hit
CAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAAA4220.12821369759796802No Hit
TCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAA4060.1233525147506517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)