FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004838155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387252
Sequences flagged as poor quality0
Sequence length300
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTGCTCATACACTTCCTGTGTACTTTTATGAATTTCAACATAGAAC18015046.52009544172787No Hit
CTCTGGGTAGTCACCAACGGTATGTGTGTTATCTCCTTCTTAGTGCAGGT17810045.9907243861878No Hit
CTGGGTAGTCACCAACGGTATGTGTGTTATCTCCTTCTTAGTGCAGGTCG23430.605032382014812No Hit
TCTGGGTAGTCACCAACGGTATGTGTGTTATCTCCTTCTTAGTGCAGGTC6010.1551960996973547No Hit
TTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAG4570.11801101091795524No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACTGTTATCTCGTAT4150.10716536002396372TruSeq Adapter, Index 4 (97% over 37bp)
GTCGTGCTCATACACTTCCTGTGTACTTTTATGAATTTCAACATAGAACA4040.10432483240887071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)