FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004838156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387252
Sequences flagged as poor quality0
Sequence length300
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTGCTCATACACTTCCTGTGTACTTTTATGAATTTCAACATAGAAC17889746.19653352339046No Hit
CTCTGGGTAGTCACCAACGGTATGTGTGTTATCTCCTTCTTAGTGCAGGT17544345.30460785225125No Hit
CTGGGTAGTCACCAACGGTATGTGTGTTATCTCCTTCTTAGTGCAGGTCG16220.4188487083346245No Hit
TCTGGGTAGTCACCAACGGTATGTGTGTTATCTCCTTCTTAGTGCAGGTC4480.11568694286924278No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4460.11517048330286221Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)