FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004838158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291578
Sequences flagged as poor quality0
Sequence length300
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGA13330045.71675503638821No Hit
TTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAG13289045.57614086110749No Hit
TTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGA18980.6509407431287683No Hit
GAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGAT10950.3755427364204432No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4140.14198602089320866Illumina Single End PCR Primer 1 (100% over 50bp)
TCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAA3860.1323831016057453No Hit
CAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAAA3840.1316971787994979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)