FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004838171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283039
Sequences flagged as poor quality0
Sequence length300
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGA13269446.8818784690449No Hit
TTTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAG13207146.661767459608036No Hit
TTCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGA10720.37874639183999376No Hit
GAGACCATGGACTGACGACATGTGTACTCACGCTTACAGCTCCAGCTGAT8140.28759287589342813No Hit
CAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAAA3570.12613102787955016No Hit
TCAGGTAGTTCATAATGCAATCATAATAGAGATGACTAATTTAGGAAGAA3530.12471779507417706No Hit
CGTCGTGCTCATACACTTCCTGTGTACTTTTATGAATTTCAACATAGAAC2930.10351930299358038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)