FastQCFastQC Report
Sat 25 May 2024
EGAF00004838269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147233981
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63643594.322615578804461Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63439784.308772986312174Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG24045741.6331651047321747Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGATGAGATGGCGAAATTCAA6608430.4488386414003164Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGA4222960.2868196574811082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCAC2238460.15203419650793795Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGCGTCCTCTGGCTCCAGGC1767930.12007622071972639Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1716040.116551898437087Clontech SMART CDS Primer II A (96% over 25bp)
AAGTAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1639260.11133706966736164No Hit
ACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTG1599700.10865018993135832Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGCGGCGTCCTCTGGCTCCAGGC1546210.10501719708305651Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCA1517430.10306248528320373Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)