FastQCFastQC Report
Sat 25 May 2024
EGAF00004838271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157174242
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61785003.930987623277356Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56320953.5833447824103395Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG28213971.7950759387151998Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGA9170910.5834868285860733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGATGAGATGGCGAAATTCAA5065650.32229517607598834Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCAC2420950.15402969145542308Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1772770.11279010971785058Clontech SMART CDS Primer II A (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTG1691930.10764677331798425Clontech Universal Primer Mix Long (96% over 26bp)
AAGTAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1672630.1064188367455273No Hit
AAGCAGTGGTATCAACGCAGAGTACGCGGCGGCGTCCTCTGGCTCCAGGC1595030.10148164099305788Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)