FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004838272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157174242
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAA38664892.460001683990943Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT14432930.9182757821093867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1993650.1268433029885393Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGATGAGATGGCGAAATTCAACTACGAGCTTACTGTACAGTCTGAG1795500.11423627543245922Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTTCTATA1693610.10775366106107896Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)