FastQCFastQC Report
Mon 13 May 2024
EGAF00004838273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105570691
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28595092.708620141550461Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26569642.516762914813165Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG20367701.929294940392121Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGA5962980.5648329042385447Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGATGAGATGGCGAAATTCAA3695060.3500081286765472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGGATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2488450.23571409606478752Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGGAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2242620.21242827708686682No Hit
AAGCAGTGGGATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG1779680.16857709115496838Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCAC1560960.14785921975257318Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGNATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1511390.14316378775999486Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGNAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1188950.11262121984216246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)