FastQCFastQC Report
Mon 13 May 2024
EGAF00004838274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105570691
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59131425.6011208641231685Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG45111594.273116863467343Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39133013.706806276374567Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGA11209561.0618060650943357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGATGAGATGGCGAAATTCAA2771200.2624970977977212Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGAAAGCAGTGGTATCAACGCAG2115910.2004258928266369Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGAAGCAGTGGTATCAACGCAGA2067710.1958602317001032Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1162960.11015936231770994Clontech SMART CDS Primer II A (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGAGAATGTTTTCATGTTAC1109540.10509924577456825Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGTAAGCAGTGGTATCAACGCAG1100520.10424484197039119Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)