FastQCFastQC Report
Tue 14 May 2024
EGAF00004838275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126271863
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36765112.911583715209777Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34560252.736971576953767Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG27826632.203707883837906Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGGCGCATCTCGTA7752470.6139507104603343Illumina Paired End Sequencing Primer 2 (100% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGA7478270.5922356590240535Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCAC3194570.2529914364215883Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGATGAGATGGCGAAATTCAA3077390.24371145929794352Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTG2202240.17440464943484676Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCG2094480.16587068173691236Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTG1897050.1502353695375509Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCT1714620.13578797043645424Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGT1682140.13321574260767818Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGT1673390.13252279330035702Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACT1652170.13084229223734506Clontech Universal Primer Mix Long (96% over 26bp)
CCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGAT1577160.12490193480395549Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCC1572000.12449329269815239Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1427190.1130251796475039Clontech SMART CDS Primer II A (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGAAAGCAGTGGTATCAACGCAG1313430.10401604671026354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGAAGCAGTGGTATCAACGCAGA1287000.10192294383112095Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)