FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004838275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126271863
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAA19798961.5679629277347402Clontech Universal Primer Mix Long (96% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7371640.5837911807795217Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT6782620.5371442092368591Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATAC2041780.16169714705167532Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCT2016110.15966423176951147Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTA1753830.13889317527531847Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGT1587440.12571605124729965Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCC1564040.12386290681400654Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGC1532490.12136432959732286Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGC1508650.11947633971314735Clontech Universal Primer Mix Long (96% over 26bp)
CCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCGTTGATACCACTGCTTCCCGCGTACTCTGCG1463710.11591735207074597Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)