FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004838276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126271863
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAA46460453.6793984737518284Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT11692670.9259917231125354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGAAGCAGTGGTATCAACGCAGAGTACGCGGAAGCAGTGGTATCAACG1872470.14828877594052764Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAGAGTACGCGGAAAGCAGTGGTATCAA1867560.14789993238636226Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGTAAGCAGTGGTATCAACGCAGAGTACGCGGTAAGCAGTGGTATCAA1633190.12933918619700732Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1585610.12557112584931135Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)