FastQCFastQC Report
Sat 25 May 2024
EGAF00004838277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146793307
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41777802.846028940542909Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTAACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24620671.6772338264713935Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCNACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15466111.0535977638272023Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG15238991.0381256687677185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTAACNACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7888100.5373610119703891No Hit
AAGCAGTGGTATCNACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG5743870.3912896382939312Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGATGAGATGGCGAAATTCAA2347870.15994394076836216Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGA2306060.15709571826731855Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGTGGCGGCGACGACCCATTC1835640.1250492980582555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAACATATCTCGTA1601990.10913235982891237Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGCAGTGGTATCGACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1519730.10352856210263048Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)