FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004838289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60746302
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT5475690.9014030187384904Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAA2536230.41751183471217723Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1649590.2715539787096834Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)