FastQCFastQC Report
Wed 22 May 2024
EGAF00004838302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155383979
Total Bases11.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148633729.565575611884672Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG8507160.5474927373304039Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2661510.17128599853914153Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGGTGCGTCGGGTCTGCGAGA2020780.1300507306483637Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)