FastQCFastQC Report
Wed 22 May 2024
EGAF00004838303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161815469
Total Bases12.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97463176.023105862641599Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGAAAATCTCGTA13256490.8192350262878761Illumina Paired End PCR Primer 2 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG5829230.36023935387784217Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1861800.11505698506488275Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)