FastQCFastQC Report
Sat 25 May 2024
EGAF00004838307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160526965
Total Bases12 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85724405.340186927473525Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGATAGATCTCGTA10097240.6290058495779821Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG4633720.28865679980930303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1730950.1078292360414339Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)