FastQCFastQC Report
Mon 13 May 2024
EGAF00004838313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132440504
Total Bases9.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92306736.969675228659655Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTA51143183.8615966003874465Illumina Paired End PCR Primer 2 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG5604620.4231802077708795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTA1996590.1507537301428572Illumina Paired End PCR Primer 2 (97% over 37bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1994570.15060120882656863Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)