FastQCFastQC Report
Mon 13 May 2024
EGAF00004838315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129796736
Total Bases9.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73766695.683246919244564Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTA66199785.100265387259045Illumina Paired End Sequencing Primer 2 (100% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTA2715530.20921404371832586Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)