FastQCFastQC Report
Mon 13 May 2024
EGAF00004838321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128771588
Total Bases9.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108624368.43542909480933Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTA34218672.657315214595319Illumina Paired End Sequencing Primer 2 (100% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG9007090.6994625242953437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2469640.19178454178883Clontech SMART CDS Primer II A (100% over 25bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTA1695920.13169985913352253Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)