FastQCFastQC Report
Mon 13 May 2024
EGAF00004838323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114521614
Total Bases8.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76119076.646699023993846Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTA46895324.094888149236178Illumina Paired End PCR Primer 2 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG3298990.288067019383782Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTA1962170.17133621606136287Illumina Paired End PCR Primer 2 (97% over 37bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1311130.11448755865421177Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)