FastQCFastQC Report
Sat 25 May 2024
EGAF00004838327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135892762
Total Bases10.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110652158.142608066204438Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTA36797102.7078042611276087Illumina Paired End Sequencing Primer 2 (100% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG2453910.1805769464013102Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2109080.1552017906590198Clontech SMART CDS Primer II A (100% over 25bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTA1931660.14214590766798899Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGATGGCACATGCAGCGCAAGT1448750.10660979868817443Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)