FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004838327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135892762
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30280852.228290127770013Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3992470.29379563276519466Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAA1863960.13716403821419126Clontech Universal Primer Mix Long (96% over 26bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1577230.11606431253490897Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC1373900.10110177906311153Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT1368940.10073678537787023Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)