FastQCFastQC Report
Sun 12 May 2024
EGAF00004838329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104872243
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81113917.734545164634269Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACAGCATCTCGTA49180084.6895230418596086Illumina Paired End Sequencing Primer 2 (100% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACAGCATCTCGTA2298920.21921148382418026Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG1809850.17257664642492676Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)