FastQCFastQC Report
Sat 25 May 2024
EGAF00004838341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157205692
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97718976.215994392874783Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGCGAATCTCGTA38531972.4510543803973714Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG3670940.23351190108307274Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGCGAATCTCGTA1672090.10636319707813123Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)