FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004838343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134530582
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA44152743.281985355567703Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2939470.21849827424369578Clontech SMART CDS Primer II A (100% over 26bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1560520.1159974168550018Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)