FastQCFastQC Report
Sat 25 May 2024
EGAF00004838347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148933163
Total Bases11.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84610125.681079908307594Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCCTAATCTCGTA45235163.0372792122866548Illumina Paired End PCR Primer 2 (97% over 39bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCCTAATCTCGTA1934870.12991532315740853Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)