FastQCFastQC Report
Sat 25 May 2024
EGAF00004838349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142992904
Total Bases10.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92775276.488103073981908Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAAACCATCTCGTA42294482.9578027172593124Illumina Paired End Sequencing Primer 2 (100% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG1914930.1339178341325245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAAACCATCTCGTA1695020.11853874930744816Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)