FastQCFastQC Report
Wed 22 May 2024
EGAF00004838351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157469428
Total Bases11.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98521566.256551589175772Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCGAAATCTCGTA51466923.268375369979753Illumina Paired End PCR Primer 2 (97% over 39bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCGAAATCTCGTA1792650.1138411450888105Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG1604740.10190803512666598Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)