FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004838359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127031737
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA42025143.308239420515836Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2793520.21990725042199494Clontech SMART CDS Primer II A (100% over 26bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2098320.16518076896012215Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA1993040.15689307625542426Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)