FastQCFastQC Report
Sat 25 May 2024
EGAF00004838361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147372449
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95559376.484208591797236Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCTCCATCTCGTA36481432.4754579466885294Illumina Paired End PCR Primer 2 (97% over 41bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCTCCATCTCGTA1806710.12259482774829913Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG1744770.11839187119703765Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)