FastQCFastQC Report
Sat 25 May 2024
EGAF00004838371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153976315
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111091227.214825215163774Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGGCCATCTCGTA31136572.022166201340771Illumina Paired End PCR Primer 2 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACGCGGGAAGCAGTGGTATCAACGCAG1695120.11008965891929547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGGCCATCTCGTA1544580.10031283058046947Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)