FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13609913
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGC18100.025.746869
TTACGAG18300.025.1431268
GTTTACG19850.025.111466
CGAGCTA18050.025.0011511
GCCCTAA38400.023.89334952
CCTAAGC40150.023.2188954
AACGCTA36600.023.0479523
ACGCTAC38000.022.35421624
CCCTAAG41500.022.25031353
CTACACG38600.021.62897157
GCTAGTC21050.021.29792214
TTTACGA24600.020.862197
CGCTACC40550.020.58477425
CTAAGCT47500.019.99710355
AGCCCTA53300.019.317551
GTCGGTT14900.019.2677231
CTAGTCT25000.019.23062315
AAAACGC44300.019.10838121
ACTATCG10450.018.349758
CCGCGTT21150.018.13206759
Produced by FastQC (version 0.11.5)