FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18075639
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT298880.16534961779221194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA34550.018.52472323
CTACACG33750.018.35630657
ACTATCG12300.017.5090458
ACGCTAC36500.017.45443324
GCCCTAA27100.017.09148452
GTCGGTT21250.016.9907551
CGCTACC37500.016.83172225
GTCCGCT23050.016.8194641
GTTCCTA34800.016.27274515
GCACTAC33950.015.89854425
CCTACTC35950.015.75219818
GTCCGTT15300.015.4742761
AGTTACC36550.015.33527634
CCCAGAT93050.015.3300091
GTCCGGT20400.015.2324911
CCCGTAT16750.014.8414461
CGAGGCG36050.014.40248954
CCTTACG15100.014.26375241
AAAACGC45700.013.87589821
CTCGGTT61350.013.5551241
Produced by FastQC (version 0.11.5)