FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22832450
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT284130.12444131050325304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA53850.022.18512752
GTTCCTA46850.020.8393615
CCTAAGC60500.020.33123654
GTCCGTT23100.019.962611
GTCGGTT28950.019.1961441
CCCTAAG62750.019.17924153
CTACACG59100.019.16596257
AACGCTA60800.018.92083523
ACGCTAC61750.018.91668924
AGTTACC52850.018.75607934
CGCTACC63350.018.01985225
CCTACTC55900.017.83490818
CCCGTAT21950.017.3724021
CTAAGCT70450.017.33414555
GCCCTAT37250.017.26295753
AGCCCTA78650.016.76609251
AAAACGC69900.016.3729621
CGAGGCG41300.016.14140954
CAACGTT114800.016.05846815
GCACTAC43450.015.95383125
Produced by FastQC (version 0.11.5)