FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21741910
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT255600.11756096865454782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA53350.028.74907923
ACGCTAC55650.027.9320924
CGCTACC57600.026.42324825
CTACACG59000.025.80058957
AAAACGC65900.023.5426521
GAAAACG70450.021.85468320
GCCCTAA49600.021.82862352
GCCGGTT54600.020.7095451
TACACGT75050.020.4008258
AAACGCT76950.020.20026222
TCACGCT81250.020.1837778
CCTAAGC54400.020.06461354
CCGGTTT52650.020.0556012
TTCACGC84800.019.512737
CCCTAAG56250.019.35226253
ACACGTT80400.019.04207459
GCTCTAC81200.019.00087454
TTTCGGT83250.018.39166547
CACGCTC88300.018.2716459
GTTCCTA38650.017.39948515
Produced by FastQC (version 0.11.5)