FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18013198
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT285840.15868364962179396No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC231230.12836698958175002No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT209710.11642019368243219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA61250.027.40555252
CCCTAAG65400.025.84608553
CCTAAGC66150.025.59756954
AACGCTA64650.024.31701323
ACGCTAC65150.024.1306624
CTACACG65650.023.95033557
CGCTACC67650.023.23904425
CTAAGCT75700.022.3675455
TACGAGC16900.021.8156269
TAAGCTC79650.021.48091956
AGCCCTA84250.021.2951
AAAACGC74000.021.20449321
GTTTACG18050.021.0793326
TTCACGC77250.021.037667
TACACGT75550.020.92927758
CCGGCTT83300.020.8198052
GAAAACG76800.020.6618420
TTCAACG83550.020.47509213
TCACGCT79150.020.4581228
CCCGTAT15250.020.3771321
Produced by FastQC (version 0.11.5)