FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18601475
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC236520.12715120709513628No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT219920.1182271835969997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA64950.026.43493552
CCTAAGC69200.024.8961354
CCCTAAG70200.024.54148353
CTAAGCT79600.021.79125855
AGCCCTA93050.020.19584851
TAAGCTC86450.020.03079456
AACGCTA32600.018.63757723
CAACGTT130800.018.55798315
CGTTGTG130350.018.5315418
GCCCTAT40350.018.4239253
TTCAACG95900.018.1456313
ACGTTGT134950.018.14027617
AACGTTG134200.018.0878116
ACGCTAC33750.017.91556224
AAGGGAC138900.017.49841327
CGCTACC35000.017.19149425
CTACACG35850.016.78677257
ACTGTAC103750.016.77660632
GTCCGGT19950.016.7656441
CCCGTAT14700.016.309941
Produced by FastQC (version 0.11.5)